Phlebotomy requisitions, lab orders, chain-of-custody forms, and NHA CPT and ASCP PBT exam questions all assume you know the abbreviations cold. This is a complete reference covering every tube, test, procedure, safety, and regulatory abbreviation you'll encounter in training, on the exam, and on the job.
How Abbreviations Are Tested on the Certification Exam
The exam doesn't ask you to define SST in isolation. It puts the abbreviation in a scenario: "A physician orders a CMP and CBC STAT. What tubes do you collect and in what order?" You need to know CMP runs on serum (gold SST), CBC runs on EDTA (lavender), what STAT means, and the correct order of draw, all from a single question stem. This reference organizes abbreviations so you can learn them alongside their clinical context, not just as a list to memorize.
Tube and Collection System Abbreviations
| Abbreviation |
Full Name |
Clinical Context |
| ETS | Evacuated Tube System | BD Vacutainer and equivalent closed-system collection. The vacuum in each tube draws a predetermined blood volume. |
| SST | Serum Separator Tube | Gold/tiger top with clot activator and thixotropic gel. Produces serum after centrifugation. Most common tube in outpatient chemistry labs. |
| PST | Plasma Separator Tube | Mint green with lithium heparin and gel. Produces plasma. Faster results than SST because no clot time required. |
| EDTA | Ethylenediaminetetraacetic acid | Anticoagulant in lavender and pink tubes. Permanently chelates calcium to prevent clotting. K2 EDTA is the current standard form. |
| SPS | Sodium Polyanethol Sulfonate | Yellow blood culture tube additive. Neutralizes antibiotics, inhibits complement and phagocytosis to preserve organisms for culture. |
| ACD | Acid Citrate Dextrose | Yellow top (different from SPS). Used for HLA typing, paternity testing, DNA studies. Rarely used in routine collection. |
| NaF | Sodium Fluoride | Glycolytic inhibitor in gray tubes. Blocks enolase to prevent glucose metabolism. Preserves glucose for up to 24 hours. |
| KOx | Potassium Oxalate | Secondary anticoagulant in gray tubes. Pairs with NaF. Chelates calcium to prevent clotting. |
| Na Citrate | Sodium Citrate | Anticoagulant in light blue (3.2%) and black (3.8%) tubes. Reversibly chelates calcium. Light blue = coagulation; black = Westergren ESR only. |
| Li Hep | Lithium Heparin | Anticoagulant in green tubes. Inhibits thrombin and factor Xa. Cannot be used for coagulation testing or lithium drug levels. |
Hematology Test Abbreviations
| Abbreviation |
Full Name |
Tube / Notes |
| CBC | Complete Blood Count | Lavender (EDTA). Includes WBC, RBC, Hgb, Hct, MCV, MCH, MCHC, RDW, platelet count. |
| WBC | White Blood Cell count | Total leukocyte count. Elevated in infection, inflammation, leukemia. Part of CBC. |
| RBC | Red Blood Cell count | Total erythrocyte count. Part of CBC. |
| Hgb / Hb | Hemoglobin | Oxygen-carrying protein in RBCs. Decreased in anemia and blood loss. |
| Hct / PCV | Hematocrit / Packed Cell Volume | Percentage of blood volume occupied by RBCs. Roughly 3x the hemoglobin value. |
| H&H | Hemoglobin and Hematocrit | Common combined order. Both drawn in lavender tube as part of CBC. |
| MCV | Mean Corpuscular Volume | Average RBC size. Low = microcytic (iron deficiency). High = macrocytic (B12/folate deficiency). |
| MCH | Mean Corpuscular Hemoglobin | Average hemoglobin content per RBC. Part of CBC indices. |
| MCHC | Mean Corpuscular Hemoglobin Concentration | Average hemoglobin concentration relative to cell volume. Decreased in iron deficiency anemia. |
| RDW | Red Cell Distribution Width | Variation in RBC size. Elevated RDW suggests mixed deficiency (iron + B12, for example). |
| Plt | Platelet count | Thrombocytes. Part of CBC. Low = thrombocytopenia. |
| MPV | Mean Platelet Volume | Average platelet size. Large platelets suggest increased platelet production (as in ITP). |
| Diff | Differential | WBC differential count: percentage of each leukocyte type (neutrophils, lymphocytes, monocytes, eosinophils, basophils). |
| Segs / Polys / PMN | Segmented neutrophils / Polymorphonuclear cells | Mature neutrophils. 50-70% of WBC differential. First responders to bacterial infection. |
| Bands | Band neutrophils | Immature neutrophils. Elevated bands ("left shift") indicates acute infection or stress on bone marrow. |
| Lymphs | Lymphocytes | 20-40% of WBC differential. B and T cells. Elevated in viral infections and some leukemias. |
| Monos | Monocytes | 2-8% of WBC. Largest leukocyte. Mature into macrophages in tissues. |
| Eos | Eosinophils | 1-4% of WBC. Elevated in allergic conditions and parasitic infections. |
| Basos | Basophils | 0-1% of WBC. Least common. Release histamine in allergic and inflammatory reactions. |
| ESR | Erythrocyte Sedimentation Rate | Black tube (3.8% sodium citrate, Westergren method). Non-specific inflammatory marker. Rate RBCs settle in 1 hour. |
| Retic | Reticulocyte count | Lavender tube. Immature RBCs. Elevated in hemolysis or blood loss (bone marrow is producing more RBCs). |
| HbA1c / A1C | Glycosylated hemoglobin | Lavender tube. Reflects average blood glucose over 2-3 months. Diabetes threshold: 6.5%. |
Chemistry and Metabolic Panel Abbreviations
| Abbreviation |
Full Name |
Tube / Notes |
| BMP | Basic Metabolic Panel | Gold SST or green. Sodium, potassium, chloride, CO2, BUN, creatinine, glucose, calcium. |
| CMP | Comprehensive Metabolic Panel | Gold SST. BMP + total protein, albumin, bilirubin, ALP, AST, ALT. |
| BUN | Blood Urea Nitrogen | Waste product from protein metabolism. Elevated in kidney disease and dehydration. |
| Cr / Creat | Creatinine | Muscle metabolism waste. More reliable kidney marker than BUN. Stable regardless of diet. |
| Na | Sodium | Primary extracellular cation. Hyponatremia and hypernatremia both have neurological consequences. |
| K | Potassium | Primary intracellular cation. EDTA carryover and hemolysis both falsely elevate potassium. Critical cardiac arrhythmia risk at extremes. |
| Cl | Chloride | Primary extracellular anion. Part of electrolyte panel. |
| CO2 / HCO3 | Bicarbonate / Carbon Dioxide | Acid-base balance marker on electrolyte panels. Not the same as PCO2 from an ABG. |
| Ca | Calcium (total) | Falsely lowered by EDTA or citrate contamination. Critical for cardiac and neuromuscular function. |
| Mg | Magnesium | Also affected by EDTA carryover. Important for cardiac rhythm and muscle function. |
| Phos | Phosphorus / Phosphate | Falsely elevated by hemolysis (released from RBCs). Important in kidney disease and electrolyte balance. |
| Gluc / FBG / FBS | Glucose / Fasting Blood Glucose / Fasting Blood Sugar | Gray tube for accuracy. Without fluoride, glucose drops 5-7 mg/dL per hour at room temperature. Diabetes threshold: fasting 126 mg/dL or higher on two occasions. |
| GTT / OGTT | Glucose Tolerance Test / Oral Glucose Tolerance Test | Gray tubes at timed intervals after glucose challenge. Used to diagnose gestational diabetes and Type 2 DM. |
| LFTs | Liver Function Tests | Gold SST. Includes AST, ALT, ALP, GGT, total bilirubin, direct bilirubin, albumin, total protein. |
| AST | Aspartate Aminotransferase | Liver enzyme. Also elevated in muscle injury. Falsely elevated by hemolysis (high concentration in RBCs). |
| ALT | Alanine Aminotransferase | More liver-specific than AST. Primary marker for hepatocellular damage. |
| ALP | Alkaline Phosphatase | Elevated in liver disease and bone disorders. Also elevated physiologically in growing adolescents and pregnancy. |
| GGT | Gamma-Glutamyl Transferase | Sensitive marker for liver disease, especially alcohol-related. Elevated in biliary obstruction. |
| T. Bili / D. Bili | Total Bilirubin / Direct Bilirubin | Protect from light during transport. Falsely decreased by light exposure. Elevated in liver disease and hemolysis. |
| Alb | Albumin | Major plasma protein. Decreased in malnutrition, liver disease, kidney disease (nephrotic syndrome). |
| LDH | Lactate Dehydrogenase | Non-specific tissue injury marker. Falsely elevated by hemolysis. Present in high concentration in RBCs. |
| TSH | Thyroid Stimulating Hormone | Gold SST. Primary thyroid screening test. Elevated = hypothyroidism. Decreased = hyperthyroidism. |
| T3 / T4 / FT4 | Triiodothyronine / Thyroxine / Free T4 | Gold SST. Thyroid hormones. Used with TSH for complete thyroid evaluation. |
| HDL / LDL | High-Density Lipoprotein / Low-Density Lipoprotein | Gold SST. Lipid panel. Higher HDL is protective. Higher LDL increases cardiovascular risk. |
| TG / Trig | Triglycerides | Fasting specimen required for accurate triglyceride measurement. Part of standard lipid panel. |
| CRP / hsCRP | C-Reactive Protein / High-Sensitivity CRP | Gold SST. Non-specific inflammatory marker. hsCRP is used for cardiac risk stratification at low concentrations. |
| UA | Urinalysis | Urine specimen. Random or clean-catch midstream. Chemical dipstick + microscopic if indicated. |
| C&S | Culture and Sensitivity | Microbiological culture to identify organism + sensitivity testing to guide antibiotic selection. |
Cardiac Biomarker Abbreviations
| Abbreviation |
Full Name |
Clinical Context |
| Trop I / Trop T | Troponin I / Troponin T | Gold SST. Current standard for MI diagnosis. Rises 3-6 hours post-MI, peaks at 12-24 hours, elevated for days. Most sensitive and specific cardiac marker available. |
| CK-MB | Creatine Kinase MB isoenzyme | Gold SST. Peaks at 12-24 hours, returns to baseline within 24-48 hours (earlier normalization than troponin makes it useful for reinfarction detection when troponin remains elevated). Used for reinfarction detection when troponin stays elevated from initial MI. Not as specific as troponin. |
| CK / CPK | Creatine Kinase / Creatine Phosphokinase | Elevated in muscle injury, rhabdomyolysis, and MI. Total CK is not cardiac-specific; CK-MB isoenzyme is. |
| BNP / NT-proBNP | B-type Natriuretic Peptide / N-terminal proBNP | EDTA tube (lavender) for BNP, gold SST for NT-proBNP. Elevated in heart failure. Used to differentiate cardiac from pulmonary causes of dyspnea. |
| D-dimer | D-dimer fibrin degradation product | Light blue (citrate) tube. Elevated in DVT, PE, DIC. Normal D-dimer is useful to rule out clot when pre-test probability is low. |
Coagulation Test Abbreviations
| Abbreviation |
Full Name |
Clinical Context |
| PT | Prothrombin Time | Light blue (3.2% sodium citrate). Measures extrinsic clotting pathway. Elevated in warfarin therapy, liver disease, vitamin K deficiency. |
| INR | International Normalized Ratio | Standardized PT result. Therapeutic warfarin range is typically 2.0-3.0. Allows comparison across different laboratory reagents. |
| PTT / aPTT | Partial Thromboplastin Time / Activated PTT | Light blue tube. Measures intrinsic clotting pathway. Used to monitor unfractionated heparin therapy. Elevated in hemophilia A and B. |
| Fibrinogen | Fibrinogen (Factor I) | Light blue tube. Decreased in DIC, liver failure. Consumed in massive clot formation. |
| DIC | Disseminated Intravascular Coagulation | Pathological activation of both clotting and fibrinolysis simultaneously. Associated with elevated D-dimer, elevated PT/PTT, decreased fibrinogen and platelets. |
| DVT | Deep Vein Thrombosis | Blood clot in deep vein, usually leg. Risk of pulmonary embolism. D-dimer used in workup. |
| PE | Pulmonary Embolism | Blood clot in pulmonary vasculature. D-dimer elevated but non-specific. CT angiography confirms. |
Special Collections and ABG Abbreviations
| Abbreviation |
Full Name |
Context |
| ABG | Arterial Blood Gas | Arterial collection (usually radial artery). Measures pH, PO2, PCO2, HCO3, O2 saturation. Transport on ice. Allen's test required first. |
| pH | Potential of Hydrogen | ABG. Acid-base balance. Normal 7.35-7.45. Below 7.35 = acidosis. Above 7.45 = alkalosis. |
| PO2 / PCO2 | Partial pressure of oxygen / carbon dioxide | ABG. PO2 measures oxygenation. PCO2 reflects ventilation. Transport on ice immediately. |
| SpO2 | Peripheral oxygen saturation (pulse oximetry) | Non-invasive. Measures hemoglobin oxygen saturation via light sensor. Not the same as PO2 from ABG. |
| BC / BCx | Blood Culture | Yellow SPS tube or directly inoculated aerobic/anaerobic bottles. Collected first, before all other tubes. |
| BAC | Blood Alcohol Concentration | Gray tube. Non-alcohol antiseptic (chlorhexidine or povidone-iodine) required. Alcohol swab invalidates forensic specimens. |
| TDM | Therapeutic Drug Monitoring | Gold SST. Timing is critical. Trough = just before next dose. Peak = collected at specific post-dose interval. |
| Trough | Trough level | Minimum drug concentration. Collected immediately before the next scheduled dose. |
| Peak | Peak level | Maximum drug concentration. Collected at a drug-specific interval after dose administration. |
| POC | Point of Care | Testing performed at or near the patient (bedside, ER, clinic) rather than the central lab. Faster results, less precision than automated analyzers. |
Pre-Analytical and Quality Control Abbreviations
| Abbreviation |
Full Name |
Context |
| QNS | Quantity Not Sufficient | Specimen rejected because volume is inadequate to run the ordered tests. Requires recollection. |
| H | Hemolysis | RBC rupture releasing intracellular contents into plasma/serum. Pink or red discoloration. Falsely elevates K, LDH, AST, phosphorus. |
| L | Lipemia | Milky or turbid plasma from elevated triglycerides. Can interfere with spectrophotometric assays. |
| I | Icterus | Yellow plasma from elevated bilirubin. Can interfere with colorimetric assays. Specimen must be protected from light. |
| HLI Index | Hemolysis, Lipemia, Icterus Index | Automated assessment of specimen quality. Flags specimens where any of the three conditions may interfere with results. |
| TAT | Turnaround Time | Time from specimen collection to reported result. STAT and routine specimens have different TAT expectations. |
| QC | Quality Control | Process of testing known control materials to verify instrument accuracy. Required before patient testing begins. |
| QA | Quality Assurance | Broader program to ensure overall laboratory quality, including pre-analytical, analytical, and post-analytical phases. |
| SOP | Standard Operating Procedure | Written, step-by-step procedural instructions. Required by CLIA for all laboratory procedures. |
Anatomical and Procedural Abbreviations
| Abbreviation |
Full Name |
Context |
| AC | Antecubital | The inside of the elbow. Primary venipuncture site. Median cubital, cephalic, and basilic veins are all here. |
| IV | Intravenous | Into a vein. Never collect from an IV site unless it's the absolute last resort (2-minute pause + 2 discard tubes required). |
| PICC | Peripherally Inserted Central Catheter | Long-term IV access inserted in arm, tip in superior vena cava. Phlebotomists don't routinely collect from PICCs without specific training. |
| CVL / CVC | Central Venous Line / Central Venous Catheter | Central access device. Collection from CVLs is a specialized nursing/medical procedure, not routine phlebotomy. |
| NPO | Nil per os (Nothing by mouth) | Patient is fasting. Required for fasting glucose, lipid panel, and many surgical procedures. |
| STAT | Statim (Latin: immediately) | Urgent specimen collection and processing required. STAT specimens go to the front of the processing queue. |
| DOB | Date of Birth | Required patient identifier. Along with full name, must be confirmed before every draw. Room number and bed number are never acceptable as patient identifiers. |
| MRN | Medical Record Number | Acceptable second patient identifier along with date of birth when confirming patient identity. |
| R/O | Rule out | Clinical shorthand on requisitions. "R/O MI" means tests ordered to exclude myocardial infarction from the diagnosis. |
Safety and Regulatory Abbreviations
| Abbreviation |
Full Name |
Exam Relevance |
| OSHA | Occupational Safety and Health Administration | Enforces Bloodborne Pathogens Standard (29 CFR 1910.1030). Requires HBV vaccine, exposure control plan, and post-exposure follow-up at no cost to employee. |
| BBP | Bloodborne Pathogens | HIV, HBV, HCV are the primary three. HBV has the highest transmission risk from needlestick. OSHA BBP Standard governs all healthcare worker protections. |
| PPE | Personal Protective Equipment | Gloves required for every venipuncture. Gown, mask, and eye protection for splash risk. Employer required to provide and maintain. |
| HBV / HCV / HIV | Hepatitis B / Hepatitis C / Human Immunodeficiency Virus | Primary bloodborne pathogens. HBV: highest needlestick risk (up to 30%). HCV: intermediate (1-3%). HIV: low (0.3%). PEP for HIV must begin within 72 hours of exposure. |
| SDS | Safety Data Sheet | Replaced MSDS. Contains hazard information for chemical substances. Must be accessible to all employees in the work area. |
| CLIA | Clinical Laboratory Improvement Amendments | Federal regulations governing all human specimen testing in the US. Established standards for laboratory quality, personnel, and proficiency testing. |
| CLSI | Clinical and Laboratory Standards Institute | Publishes voluntary consensus standards including GP41 (order of draw, venipuncture), GP42 (skin puncture), and others. Both certification exams reference CLSI standards directly. |
| CAP | College of American Pathologists | Laboratory accreditation organization. More rigorous than CLIA minimum standards. |
| TJC | The Joint Commission | Accredits hospitals and healthcare organizations. Sets patient identification and safety standards that phlebotomists must follow. |
| HIPAA | Health Insurance Portability and Accountability Act | Federal law protecting patient health information (PHI). Phlebotomists cannot disclose test results or patient information to unauthorized individuals. |
| PHI | Protected Health Information | Any individually identifiable health information. Name, DOB, diagnosis, test results, and room number all qualify as PHI. |
Certification Abbreviations
| Abbreviation |
Full Name |
Notes |
| ASCP | American Society for Clinical Pathology | The most widely recognized phlebotomy certification body in the US. Board of Certification (BOC) administers the PBT exam. |
| PBT | Phlebotomy Technician (ASCP credential) | ASCP PBT certification requires clinical training hours and passing the BOC exam. Renewal requires continuing education credits. |
| NHA | National Healthcareer Association | Issues CPT (Certified Phlebotomy Technician) credential. Accepted in many states and facilities. |
| CPT | Certified Phlebotomy Technician | NHA credential. Different from the medical billing CPT code abbreviation. |
| AMT | American Medical Technologists | Issues RPT (Registered Phlebotomy Technician) credential. |
| CE | Continuing Education | Required for ASCP PBT certification renewal. Specific credit requirements set by ASCP BOC. |
| NAACLS | National Accrediting Agency for Clinical Laboratory Sciences | Accredits phlebotomy training programs. Graduates of NAACLS-accredited programs meet the educational requirement for ASCP PBT certification. |
Commonly Confused Abbreviations
These pairs appear on the certification exam specifically because they're easy to mix up:
- PT vs. PTT: PT = prothrombin time (extrinsic pathway, warfarin monitoring). PTT = partial thromboplastin time (intrinsic pathway, heparin monitoring). Both use the light blue tube. The difference is the clinical question and which pathway is being tested.
- INR vs. PTT: INR is a standardized version of the PT result. PTT is a different test entirely. Warfarin is monitored by INR. Unfractionated heparin is monitored by PTT.
- BMP vs. CMP: BMP (Basic) has 8 components. CMP (Comprehensive) adds liver panel tests. Both run on gold SST.
- ABG vs. SpO2: ABG is arterial blood collected at the wrist. SpO2 is a non-invasive pulse oximetry reading. ABG measures actual oxygen and CO2 partial pressures plus pH. SpO2 measures hemoglobin saturation only.
- ESR tube vs. coagulation tube: ESR uses black tube (3.8% citrate). Coagulation uses light blue tube (3.2% citrate). Not interchangeable.
- SPS vs. ACD: Both yellow tubes. SPS = blood cultures. ACD = HLA typing and DNA studies. Read the label.
Frequently Asked Questions
What does STAT mean in phlebotomy?
STAT comes from the Latin statim, meaning immediately. A STAT order requires urgent specimen collection and prioritized processing. STAT specimens typically have turnaround time requirements of 30-60 minutes depending on the facility and test.
What does QNS mean on a lab result?
QNS stands for Quantity Not Sufficient. The lab is indicating that the specimen volume collected was inadequate to run the ordered tests. A redraw is required. Common causes include premature needle withdrawal, difficult stick with slow fill, or requesting too many tests on a single tube.
What is the HLI index?
The HLI index stands for Hemolysis, Lipemia, and Icterus. Automated analyzers assess each specimen for these three conditions on a numeric scale. A high H index (hemolysis) suggests the specimen was improperly collected or processed. A high L index (lipemia) usually reflects the patient's clinical state. A high I index (icterus) reflects elevated bilirubin.
What does NPO mean and why does it matter for phlebotomy?
NPO means "nil per os," Latin for nothing by mouth. A patient who is NPO has been fasting. This is relevant because fasting status affects glucose (fasting required for accurate fasting glucose), lipid panel (fasting required for triglycerides and LDL calculation), and some other tests. Confirm fasting status before collecting glucose tolerance test specimens.
What does the CLSI govern in phlebotomy?
CLSI (Clinical and Laboratory Standards Institute) publishes the consensus standards that govern phlebotomy practice. The most relevant for phlebotomists is GP41 (Collection of Diagnostic Venous Blood Specimens), which defines the order of draw, needle gauge recommendations, tourniquet time limits, and tube inversion requirements.